Contact an Account ManagerStandard SPE Procedure for CHROMABOND® PS-Mix
Summary
Type: SPE
Application #: 304037
Catalog#: 730310 - Chromab. columns PS-MIX, 6 ml, 900 mg
Detection:
Conditions: Column conditioning: 3 ml methanol, then 3 ml water.
(Do not let run the column dry.)
Sample aspiration: The prepared sample is passed through the column by vacuum or pressure (max. 1000 ml sample volume)
Washing : With 3 ml AcOH / water (5:95, v/v) impurities are removed.
Elution 1: Polar and basic compounds are eluated with 3 ml methanol / water (1:1, v/v) + 10% NH3
Elution 2: Polar and acidic compounds are eluated with 3 ml methanol / water (1:1, v/v) + 10% AcOH
Elution 3: Nonpolar and neutral compounds are eluated with 3 ml methanol / acetone (1:5, v/v)
Authors: SPE department
Source: Macherey-Nagel
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